correlation of multi-drug resistance, integron and blaesbl gene carriage with genetic fingerprints of extended-spectrum β-lactamase producing klebsiella pneumoniae
نویسندگان
چکیده
conclusions: our findings suggest the possible co-carriage of some blashv genes and aris on the same plasmids harboring the mdr genes. possible role of integrons in dissemination of esbl-encoding blashv genes among esbl-producing k. pneumoniae nosocomial isolates may be inferred. background: some genetic and phenotypic variables are associated among distinct microbial populations. objectives: the associations between multi-drug resistance (mdr) phenotypes, prevalence of antibiotic resistance integrons (aris), blashv, blatem and blactx-m gene carriage and genetic fingerprints of random amplified polymorphic dna (rapd), confirmed by pulsed field gel electrophoresis (pfge), were investigated among extended-spectrum β-lactamases (esbl)-producing nosocomial isolates of klebsiella pneumoniae. materials and methods: susceptibility of 35 esbl-producing k. pneumoniae nosocomial isolates to 22 antimicrobial agents was determined. integron carriage was detected using specific primers for inti1, inti2 and inti3 genes by pcr. results: all isolates were resistant to piperacillin and susceptible to imipenem. mdr phenotype was observed in 91.4% of the isolates. class 1 integrons were detected in 21 (60%) and class 2 integrons in 3 (8.57%) of the isolates. two of the isolates carried both classes and none harbored class 3 integrons. significant correlations were observed between resistance to aminoglycosides, fluoroquinolones and sulfonamides, and between genotype groups with carriage of aris, mdr phenotype and blashv gene carriage. ari carriage was also significantly associated with mdr phenotype.
منابع مشابه
Correlation of Multi-drug Resistance, Integron and blaESBL Gene Carriage With Genetic Fingerprints of Extended-Spectrum β-Lactamase Producing Klebsiella pneumoniae
BACKGROUND Some genetic and phenotypic variables are associated among distinct microbial populations. OBJECTIVES The associations between multi-drug resistance (MDR) phenotypes, prevalence of antibiotic resistance integrons (ARIs), bla SHV, bla TEM and bla CTX-M gene carriage and genetic fingerprints of random amplified polymorphic DNA (RAPD), confirmed by pulsed field gel electrophoresis (PF...
متن کاملIntegron mediated multidrug resistance in extended spectrum beta-lactamase producing clinical isolates of Klebsiella pneumoniae
The present study describes integron mediated multiple antibiotic resistance in extended-spectrum β-lactamase producing clinical isolates of Klebsiella pneumoniae. One hundred and four clinical isolates of K. pneumoniae from two Iranian hospitals were screened for extended-spectrum β-lactamase production and susceptibility of the extended-spectrum β-lactamase producing isolates was determined t...
متن کاملprevalence of quinolone resistance among extended-spectrum β -lactamase producing uropathogenic klebsiella pneumoniae
background extended-spectrum β-lactamase (esbl) production is the major resistance mechanism to β-lactam antibiotics in enterobacteriaceae. in addition, emergence of plasmid-mediated quinolone resistance (pmqr) in esbl-producing isolates has become a global threat for treatment of these infections. objectives we investigated the association between esbl production and quinolone resistance in ur...
متن کاملPrevalence of Extended-Spectrum β-lactamase and Integron Gene Carriage in Multidrug-Resistant Klebsiella Species Isolated from Outpatients in Yazd, Iran
Background: Community-acquired infections by multidrug-resistant (MDR), extended-spectrum β-lactamase (ESBL) producing Klebsiella species (Klebsiella spp.) is of major concern worldwide. Antibiotic resistance, production of extended-spectrum β-lactamases (ESBLs), and carbapenemases, as well as the presence of classes 1, 2, and 3 integrons in outpatient isolates of Klebsiella collected from Yazd...
متن کاملPrevalence of Quinolone Resistance Among Extended-Spectrum β -Lactamase Producing Uropathogenic Klebsiella pneumoniae
BACKGROUND Extended-spectrum β-lactamase (ESBL) production is the major resistance mechanism to β-lactam antibiotics in Enterobacteriaceae. In addition, emergence of plasmid-mediated quinolone resistance (PMQR) in ESBL-producing isolates has become a global threat for treatment of these infections. OBJECTIVES We investigated the association between ESBL production and quinolone resistance in ...
متن کاملIn vitro emergence of carbapenem resistance in extended-spectrum β-lactamase-producing Klebsiella pneumoniae clinical isolates.
Klebsiella pneumoniae is thought to be the most common species producing ESBLs, and almost 50% of Klebsiella pneumoniae isolates produce ESBL in some countries [1]. Furthermore, antimicrobial co-resistance within ESBL-producing isolate communities limits the number of drugs that are effective against these strains, leaving carbapenems as the most reliable agents [2-3]. Carbapenem resistance can...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
jundishapur journal of microbiologyجلد ۷، شماره ۲، صفحات ۰-۰
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023